|Faculty of Biology, University of Latvia|
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
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Environmental and Experimental Biology
Environmental and Experimental Biology is an international print/electronic journal published four times a year, owned by a non-profit-making government-funded university. Original research papers covering all aspects of modern biology are welcome. The EEB publishes original articles, brief communications and reviews. Founded in 2003 as Acta Universitatis Latviensis ser. Biology, since 2010 EEB.
Publication is free of charge.
Teixeira da Silva T.A., Kulus D., Zhang X., Zeng S., Ma G., Piqueras A. 2016. Disinfection of explants for saffron (Crocus sativus) tissue culture.
Environmental and Experimental Biology 14: 183–198.
In vitro response of active vegetative buds of Crocus sativus L. after the surface disinfection of corms using the following procedure: wash in running tap water with 0.5% Cedepol, a detergent, and a few drops of Tween-20, transfer to 70% ethanol for 1 min then to 0.1% HgCl2 for 10 min completed with five rinses in sterile distilled water. (A) Increase in corm size on 2 µM BA + 2 µM NAA + 40 to 60 g L–1 table sugar after 12 weeks. (B) Increase in corm size on 2 µM BA + 2 µM NAA + 40 g L–1 sucrose after 12 weeks. (C) Increase in corm size and multiple thick root formation on 8.8 µM IBA + 40 g L–1 table sugar after 12 weeks. (D) Increase in corm size and germination on 2 µM BA + 2 µM NAA + 2.5 g L–1 KCl + 40 g L–1 table sugar + 30 g L–1 sucrose after 12 weeks. Figure reproduced from Quadri et al. (2010), with kind permission from Global Science Books (www.globalsciencebooks.info).
Prof. Gederts Ievinsh
University of Latvia
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