Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2003) 662: 17–24
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Environmental and
Experimental
Biology

Acta Univ Latv (2003) 662: 17–24

Orginal Article

Evaluation of the insertion-sequence-6110-based polymerase chain reaction for detection ofpathogenic mycobacteria

Viesturs Baumanis1, Inta Jansone1, Lonija Broka2, Anda Nodieva3, Mihails Bratkovskis2, Tatjana Tracevska1*
1Laboratory of Molecular Microbiology, Biomedical Research and Study Centre, University of Latvia, Rātsupītes iela 1, Rīga, LV-1067, Latvia
2State Centre of Tuberculosis and Lung Diseases, Stopiņi, Rīgas rajons, LV-2118, Latvia
3Rīga Stradiņš University, Dzirciema iela 16, Rīga, LV-1007, Latvia
*Corresponding author, E-mail: tatjanap@biomed.lu.lv

Abstract

The insertion sequence IS6110 is a mobile genetic element typical for Mycobacterium tuberculosis complex (MTC) bacteria. Due to its narrow host range, this insertion element can be used for rapid identification of pathogenic mycobacteria. The aim of this study was to evaluate the diagnostic value of the IS6110 element in comparison with common molecular and microbiological methods. A total of 170 clinical specimens from tuberculosis (TB) patients were tested for the presence of IS6110 by polymerase chain reaction (PCR). Detection of MTC by IS6110–PCR revealed the highest number of MTC positive specimens (40.6 %) in comparison to commercial LCx assay (27.6 %) and to the commonly used methods for estimating bacteria growth: BACTEC system (20.6 %) and on Lowenstein-Jensen medium (15.3 %). IS6110-PCR was shown to be a rapid and specific method for identification of the Mycobacterium tuberculosis complex. It was found to be especially useful for confi rming diagnosis in cases of smear- and culture-negative results, when the clinical expansion of tuberculosis was obvious.

Key words: Identification, insertion sequence 6110, Mycobacterium tuberculosis complex, polymerase chain reaction.

 
Acta Univ Latv (2003) 662: 17–24
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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