Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2003) 662: 51–58
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Environmental and

Acta Univ Latv (2003) 662: 51–58

Orginal Article

Changes of the secretory system of leaf epidermal and mesophyll cells during stress and programmed cell death

Turs Selga*
Laboratory of Plant Cell Biology, Faculty of Biology, University of Latvia, Kronvalda bulv. 4, Rīga, LV-1848, Latvia
*Corresponding author, E-mail: turs@lanet.lv


Comparison was made between the effects of ethylene-induced programmed cell death and leaf senescence-caused programmed cell death on the Golgi apparatus and secretory pathway in tobacco leaves. Transgenic tobacco (Nicotiana tabacum L.) plants containing jellyfish green fluorescent protein (GFP) fused to rat sialyltransferase located in Golgi bodies and endoplasmic reticulum were used. Forty-day old plants were sprayed with Ethephon at concentrations of 5 × 10-3 M or 5 × 10-2 M. We observed cells from intact leaves and leaf sections cultivated in medium. Intact control plants and sections from expanding and mature leaves cultivated in Murashige and Skoog basal medium showed a high number of vectorially moving and oscillating Golgi bodies. Programmed cell death caused by leaf senescence or induced by Ethephon treatment caused a decrease of GFP fluorescence. Golgi bodies were located in the basal part of palisade parenchyma cells in senescent leaves but Ethephon treatment caused an increase of the number of Golgi bodies in the apical part of palisade parenchyma cells.

Key words: Golgi bodies, Ethephon, ethylene, Green Fluorescent Protein, Nicotiana tabacum, programmed cell death.

Acta Univ Latv (2003) 662: 51–58
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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