Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 143–148
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Environmental and

Acta Univ Latv (2004) 676: 143–148

Orginal Article

Regulation of cytokinin response-competence by cold treatment of mature Pinus sylvestris tissues in vitro

Una Andersone1*, Gederts Ievinsh1,2
1Laboratory of Plant Tissue Culture, National Botanic Garden, Miera 1, Salaspils LV-2169, Latvia
2Department of Plant Physiology, Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Rīga LV-1586, Latvia
*Corresponding author, E-mail: invitro@nbd.apollo.lv


One of the possible reasons for lack of morphogenesis during tissue culture of mature tissues is the inability to respond to cytokinin. In the present experiments, cold incubation of pine (Pinus sylvestris L.) bud explants was used as a means to affect cytokinin response competence. Pulse treatment with cytokinins of pine bud explants cultivated at 22°C did not result in increased morphogenesis. New bud and shoot formation was achieved on pine bud explants which were pulse-treated with BAP in combination with cold incubation. It is concluded that, due to the temporary nature of juvenilization of mature pine bud explants, it is necessary to combine both physical and biochemical means to promote morphogenic competence and to achieve further development in tissue culture.

Key words: cold incubation, cytokinin response competence, morphogenesis in vitro, Pinus sylvestris.

Acta Univ Latv (2004) 676: 143–148
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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