Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 153–158
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Environmental and

Acta Univ Latv (2004) 676: 153–158

Orginal Article

Embryogenesis, callogenesis and plant regeneration from anther cultures of spring rape (Brassica napus L.)

Natalija Burbulis, Ramune Kupriene*, Liuda Žilenaite
Lithuanian University of Agriculture, Studentų 11, Akademija LT-53067, Kaunas distr., Lithuania
*Corresponding author, E-mail: ramune.kupriene@hotmail.com


Anther culture is a very important and useful tool in plant breeding for haploid production. The investigation was carried out with three spring rape cultivars, 'Trend', 'Landmark' and 'Auksiai'. Rape anthers were cultivated in modified Nitch and Nitch induction medium supplemented with 13 % sucrose and 0.4 % agarose. Embryo regeneration took place in a modified B5 Gamborg nutrient medium supplemented with 0.1 mg l-1 GA3 and 0.8 % agar. Temperature pretreatment of flower buds was at 35 °C for 72 h. After 72 h thermal shock pretreatment, the callus formation frequency differed for the tested genotypes. Thermal shock pretreatment on the callogenesis process appeared to be genotype dependent, since this treatment failed to significantly increase for 'Auksiai' and significantly decrease callus formation for the 'Landmark' and 'Trend' cultivars. Further embryo development was noted only in genotype 'Trend'. The frequency of embryo formation from anthers treated with high temperature was higher than the control. It was observed that in haploids the stomata length was reduced by 1.4 and their number in the vision field increased by approximately 1.1 in comparison with the diploid parent plants. The height of the haploid plants was 1.4 times less than parent plants. Plant regenerants were 100 % haploids.

Key words: anther culture; Brassica napus L; callogenesis; embryogenesis; nutrient media; regeneration; temperature pretreatment.

Acta Univ Latv (2004) 676: 153–158
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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