Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 159–162
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Environmental and

Acta Univ Latv (2004) 676: 159–162

Orginal Article

In vitro germination of seeds of some rare tropical orchids

Lyudmila Buyun*, Alla Lavrentyeva, Lyudmila Kovalska, Roman Ivannikov
N.N. Grishko National Botanical Garden, National Academy of Sciences of Ukraine, Timiriazevskaya 1, Kiev 01014, Ukraine
*Corresponding author, E-mail: lbuyun@i.com.ua


The objective of the study was to develop an appropriate method for in vitro seed propagation of Cattleya aclandiae Lindl., Cattleya bowringiana Veitch, Cattleya granulosa Lindl., Cattleya percivaliana O'Brien, Cattleyopsis lindenii (Lindl.) Cogn., and Dendrobium parishii Rchb.f. species, members of the Epidendroideae subfamily of the Orchidaceae family. Special attention was paid to studying the morphology of seeds, different stages of protocorms, and seedlings produced in vitro. Two seed types were been recognized according to the classification of Dressler. The highest percent of seed germination (about 100 %) was obtained on the standard Knudson medium supplemented by 2 g l-1 peptone, 50 mg l-1 potassium hummate and 1 g l-1 activated charcoal. It was shown that Dendrobium parishii had the shortest (about 300 days) period of in vitro cultivation, and Cattleyopsis lindenii had the longest (about 600 days). The formation of Cattleya seedlings, on average, took about 500 days. By this time, the most advanced seedlings had been transferred to glasshouse. The use of immature seeds capsules as a seed source shortened the reproductive cycle by approximately 2 to 2.5 months. The results of these experiments showed that all of the studied species can be effectively propagated by in vitro seed culture with the aim of ex situ biodiversity conservation.

Key words: in vitro culture, orchids, propagation, seed germination.

Acta Univ Latv (2004) 676: 159–162
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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