Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 163–166
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Environmental and

Acta Univ Latv (2004) 676: 163–166

Orginal Article

Tissue culture for elimination of lily viruses depending on explant type

Stase Dapkṻniene1*, Gitana Indrišiūnaite2, Regina Juodkaite2, Meletele Navalinskiene3, Marija Samuitiene3
1Department of Plant Physiology and Microbiology, Vilnius University, Ciurlionio 21/27, Vilnius-9 LT-10301, Lithuania
2Botanical Gardens of Vilnius University, Kairenų 43, Vilnius-40 LT-10239, Lithuania
3Institute of Botany, Žaliųjų Ežerų 49, Vilnius-21 LT-08406, Lithuania
*Corresponding author, E-mail: stase.dapkuniene@gf.vu.lt


The aim of the present work was to assess the potential of lily virus elimination by tissue culture in vitro. The medium used for explants was based on Murashige and Skoog medium. Plantlets regenerated from explants were examined by electron microscopy. Plantlets from basal parts of the petals and of basal pieces of leaves used as explants resulted in the best virus elimination. In conclusion, tissue culture is applicable for virus elimination in lily cultivars and hybrids but the success depends on the type of explants.

Key words: explant, lily in vitro, regeneration, virus elimination.

Acta Univ Latv (2004) 676: 163–166
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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