Acta Univ Latv (2004) 676: 177–182

Clonal propagation of Yucca aloifolia L.

Abstract

The optimal nutrient media and concentrations of growth regulators for obtaining Yucca aloifolia L. plants from seed culture, axillary branching and rooting of microshoots were determined. The common duration of germination of seeds of yucca in vitro on the hormone-free Monnier medium was 152 days to achieve 100 % germination. For the first time, modified Quorin and Lepover medium supplemented with 6-benzylaminopurine (1.5 mg l-1) and naphthalene acetic acid (0.06 - 0.1 mg l-1) was used for adventive shoot formation (7 to 8 shoots per explant) in Y. aloifolia epicotyl culture. The effects of indole-3-acetic acid, naphthalene acetic acid indole-3-butyric acid, 2.4- dichlorophenoxyacetic acid and its combinations on root proliferation were determined. The best rooting of microshoots of Y. aloifolia was found on Murashige and Skoog medium supplemented with 1 mg l-1 indole-3-butyric acid and light intensity 800 to 1200 lux. The rate of regenerant survival reached 87.1 % in a soil mixture of garden soil / peat / sand (2:1:1). The obtained results were useded to develop a the scheme of clonal propagation of Y. aloifolia.