Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 177–182
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Environmental and
Experimental
Biology

Acta Univ Latv (2004) 676: 177–182

Orginal Article

Clonal propagation of Yucca aloifolia L.

Pavel Karpov*
Department of Genomics and Biotechnology, Institute of Cell Biology and Genetic Engineering, NASU, Acad. Zabolotnogo 148, Kiev 03143, Ukraine
*Corresponding author, E-mail: Karpov_Pavel@univ.kiev.ua

Abstract

The optimal nutrient media and concentrations of growth regulators for obtaining Yucca aloifolia L. plants from seed culture, axillary branching and rooting of microshoots were determined. The common duration of germination of seeds of yucca in vitro on the hormone-free Monnier medium was 152 days to achieve 100 % germination. For the first time, modified Quorin and Lepover medium supplemented with 6-benzylaminopurine (1.5 mg l-1) and naphthalene acetic acid (0.06 - 0.1 mg l-1) was used for adventive shoot formation (7 to 8 shoots per explant) in Y. aloifolia epicotyl culture. The effects of indole-3-acetic acid, naphthalene acetic acid indole-3-butyric acid, 2.4- dichlorophenoxyacetic acid and its combinations on root proliferation were determined. The best rooting of microshoots of Y. aloifolia was found on Murashige and Skoog medium supplemented with 1 mg l-1 indole-3-butyric acid and light intensity 800 to 1200 lux. The rate of regenerant survival reached 87.1 % in a soil mixture of garden soil / peat / sand (2:1:1). The obtained results were useded to develop a the scheme of clonal propagation of Y. aloifolia.

Key words: growth regulators, in vitro propagation, yucca.

 
Acta Univ Latv (2004) 676: 177–182
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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