Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 183–188
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Environmental and

Acta Univ Latv (2004) 676: 183–188

Orginal Article

Tissue culture technology in conservation of threatened plant species of Latvia

Dace Kļaviņa1*, Agnese Gailīe1, Gunta Jakobsone1, Jevgēnija Ņečajeva2, Ģertrṻde Gavrilova3
1National Botanic Garden of Latvia, Miera 1, Salaspils LV-2169, Latvia
2Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Rīga LV-1586, Latvia
3Institute of Biology, University of Latvia, Miera 3, Salaspils LV-2169, Latvia
*Corresponding author, E-mail: dace.klavina@nbd.apollo.lv


To meet the requirements of the Convention on Conservation of Biological Diversity and internationally accepted botanical garden targets, the National Botanic Garden of Latvia started the elaboration of in vitro conservation methods for threatened plant species of Latvia. The objective of the present work was to develop a reliable protocol for in vitro conservation of threatened plants. Seeds and other materials of 40 threatened plant species from natural habitats were collected. In total 37 species were introduced in sterile conditions. For 20 species culture was initiated by seed germination and for six species – from shoot apex explants. Seed germination in sterile culture was not observed for 11 of experimental species. In five cases seed germination failure might be due to incomplete development of seeds. Normal organogenesis was observed in medium without hormones. In culture, the introduced species had different growth potentials in vitro. Development of germplasm conservation technique by minimal growth storage was started for species with a high multiplication rate.

Key words: conservation of biological diversity, endangered wild plants, establishment in vitro, multiplication, rooting.

Acta Univ Latv (2004) 676: 183–188
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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