Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 189–196
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Environmental and
Experimental
Biology

Acta Univ Latv (2004) 676: 189–196

Orginal Article

Development of recipient system of woody subtropical plants in vitro

Irina V. Mitrofanova*, Olga V. Mitrofanova
Department of Plant Biotechnology and Biochemistry, Nikitsky Botanical Gardens – National Scientific Center, Nikita, Yalta 98648, Crimea, Ukraine
*Corresponding author, E-mail: in_vitro@ukr.net

Abstract

The objecive of this study was to develop recipient systems of woody subtropical species and to obtain asimina (Asimina triloba Dun.), feijoa (Feijoa sellowiana Berg.), kiwi (Actinidia deliciosa (Chev.) Liang, Ferguson), persimmon (Diospyros kaki L.), and zizyphus (Zizyphus jujuba Mill.) plants through direct and indirect regeneration in vitro. The morphogenetic capacity of organs and tissues in selected woody subtropical plants was realized via five ways: activation of apical and lateral meristems; direct regeneration of plants from leaves, shoot segments and cotyledons; direct regeneration of seedlings from zygotic embryos; and direct and indirect somatic embryogenesis. High effective microshoot regeneration was induced by using direct regeneration of plants from leaves, shoot segments and cotyledons on modified Murashige and Skoog medium with 1.0 to 6.0 μM thidiazuron. The developed in vitro systems of woody subtropical plants allow us to use them for different purposes of plant biotechnology: multiplication, genetic transformation and conservation in vitro of valuable genotypes of asimina, feijoa, kiwi, persimmon and zizyphus.

Key words: Actinidia deliciosa, Asimina triloba, Diospyros kaki, Feijoa sellowiana, Zizyphus jujuba, in vitro regeneration.

 
Acta Univ Latv (2004) 676: 189–196
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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