Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 207–212
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Environmental and
Experimental
Biology

Acta Univ Latv (2004) 676: 207–212

Orginal Article

In vitro propagation of Vaccinium species

Mária Gabriela Ostrolucká1, Gabriela Libiaková1, Emília Ondrußková2, Alena Gajdoßová1
1Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, Nitra 950 07, Slovak Republic
2Department of Botany and Genetics, Faculty of Natural Sciences Constantine the Philosofer University, Nábrežie mládeže 91, Nitra 949 74, Slovak Republic
*Corresponding author, E-mail: gabriela.ostrolucka@savba.sk

Abstract

The aim of the work was direct regeneration of shoots from meristems, as well as adventitious shoot production from leaves for regeneration and effective production of highbush blueberry Vaccinium corymbosum L. and lingonberry Vaccinium vitis-idaea L. The results showed that the shoot proliferation intensity of individual cultivars differed. Therefore, optimisation of cytokinin type and concentration for each cultivar was necessary. For meristem regeneration, improved multiplication was achieved on medium with zeatin in comparison with N6-Δ2-isopentenyl adenine. Thidiazuron was effective in adventitious shoot regeneration from leaf tissue of highbush blueberry, and both thidiazuron and zeatin for lingonberry leaf tissue. Microshoot rooting was achieved on Anderson culture medium supplemented with indole-3-butyric acid or directly in peat after dipping of shoots into indole-3-butyric acid solution under ex vitro conditions.

Key words: adventitious organogenesis, meristem culture, regeneration in vitro, Vaccinium corymbosum L., Vaccinium vitis-idea L.

 
Acta Univ Latv (2004) 676: 207–212
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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