Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 231–233
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Environmental and

Acta Univ Latv (2004) 676: 231–233

Orginal Article

Plant regeneration from leaves of Cydonia oblonga cultivars

Gražina Staniene*, Vidmantas Stanys
Lithuanian Institute of Horticulture, Babtai LT-5433, Kaunas distr., Lithuania
*Corresponding author, E-mail: stanys@lsdi.lt


Regeneration experiments in vitro were started with the aim to create a transformation system for Cydonia oblonga Mill. The optimal conditions for microplant regeneration of cvs. K.11, K.16, K.19 of Cydonia oblonga were determined. Leaves were isolated from microshoots from in vitro culture and grown on Murashige and Skoog nutrient medium supplemented with inosite, vitamins, naphtilacetic acid, sucrose and various amounts of thidiazuron. It was determined that by modifying the thidiazuron concentration in nutrient medium was possible to induce morphogenesis in a sufficient frequency for transformation. The frequency of morphogenesis depended on the plant genotype. The highest output of regenerants was obtained by using Cydonia oblonga cv. K.11. The various plant genotypes required different thidiazuron concentrations in nutrient medium. A higher thidiazuron concentration was necessary (32 μM) to produce a given number of regenerants by leaves of cv. K.19 in comparison with cv. K.16. In contrast with plum trees, the morphogenesis of Cydonia oblonga in vitro was associated with callus induction.

Key words: Cydonia oblonga, plant regeneration, tissue culture.

Acta Univ Latv (2004) 676: 231–233
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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