Acta Univ Latv (2004) 676: 231–233

Plant regeneration from leaves of Cydonia oblonga cultivars

Abstract

Regeneration experiments in vitro were started with the aim to create a transformation system for Cydonia oblonga Mill. The optimal conditions for microplant regeneration of cvs. K.11, K.16, K.19 of Cydonia oblonga were determined. Leaves were isolated from microshoots from in vitro culture and grown on Murashige and Skoog nutrient medium supplemented with inosite, vitamins, naphtilacetic acid, sucrose and various amounts of thidiazuron. It was determined that by modifying the thidiazuron concentration in nutrient medium was possible to induce morphogenesis in a sufficient frequency for transformation. The frequency of morphogenesis depended on the plant genotype. The highest output of regenerants was obtained by using Cydonia oblonga cv. K.11. The various plant genotypes required different thidiazuron concentrations in nutrient medium. A higher thidiazuron concentration was necessary (32 μM) to produce a given number of regenerants by leaves of cv. K.19 in comparison with cv. K.16. In contrast with plum trees, the morphogenesis of Cydonia oblonga in vitro was associated with callus induction.