Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 243–246
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Environmental and
Experimental
Biology

Acta Univ Latv (2004) 676: 243–246

Orginal Article

Preservation of the rare terrestrial orchids in vitro

Andres Vaasa*, Viive Rosenberg
Estonian Agricultural University, Plant Biotechnological Research Centre EVIKA, Teaduse 6a, Saku 75501, Harjumaa, Estonia
*Corresponding author, E-mail: andres.vaasa@mail.ee

Abstract

The aim of this study was to establish viable tissue culture of Estonian terrestrial orchids for in vitro conservation and for final reindroduction of propagated plants to their areas. In total 28 meristems of Dactylorhiza baltica (Klinge) Orlova were excised from protocorm-like bodies and planted to modified Murashige and Skoog medium supplemented with 0.5 mg l-1 naphthaleneacetic acid and 2.0 mg l-1 N6-Δ2-isopentenyl adenine. After four weeks of culture, 60 % of excised apical meristems of D. baltica had started to grow while none of the explants originating from the lateral meristems expressed any tissue development. Semi-ripened seeds of D. ruthei (M. Schulze ex Ruthe) Soo and D. praetermissa (Druce) Soo. were sown to modified Heller, Lindemann, Norstog or Murashige and Skoog (control) medium. The current studies showed that high concentrations of microelements are necessary for germination of orchid seeds. Depending on species, germination of orchid seeds started two months after initiation. Preliminary results have been obtained regarding in vitro culture of D. baltica, D. ruthei and D. praetermissa.

Key words: orchids, preservation, regeneration, seeds, tissue culture.

 
Acta Univ Latv (2004) 676: 243–246
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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