Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2004) 676: 247–250
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Environmental and
Experimental
Biology

Acta Univ Latv (2004) 676: 247–250

Orginal Article

Agrobacterium rhizogenes-mediated transformation and regeneration of bisexual Actinidia kolomikta

Rael Vardja*, Tõnis Vardja
Institute of Experimental Biology, Estonian Agricultural University, Instituudi 11, Harku 76902, Harjumaa, Estonia
*Corresponding author, E-mail: rael19@hot.ee

Abstract

The aim of the work was to produce Actinidia kolomikta plants with modified growth and root capacity. Four highly branched root clones were obtained from leaf explants of bisexual A. kolomikta plants by Agrobacterium rhizogenes transformation. Two transformed shoot clones were regenerated from hairy roots on solid half-strength Woody Plant Medium with varying concentrations of benzyladenine (0.5 to 5.0 mg l-1). Shoot cultures of transformed plants required culture media for proliferation and rooting different from those suitable for the control. The response to exogenos hormones was modified in the transformants. Shoot proliferation required benzyladenine as the only hormone. Microcuttings from transformed in vitro plants formed dense root systems on auxinfree Murashige and Skoog medium. Leaf explants from transformed plants produced roots on hormone-free Murashige and Skoog medium, a typical response of plants regenerated from hairy roots. No rooting was observed on untransformed actinidia leaf explants under the same conditions. Transformants that had grown in the unheated greenhouse suffered from pests, and they tended to be sensitive to frost in winter.

Key words: Agrobacterium rhizogenes, bisexual Actinidia kolomikta, hairy roots, plant regeneration.

 
Acta Univ Latv (2004) 676: 247–250
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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