Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2005) 691: 129–135
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Environmental and

Acta Univ Latv (2005) 691: 129–135

Orginal Article

Organogenesis in callus cultures of Linum usitatissimum L.

Natalija Burbulis*, Ausra Blinstrubiene, Egidija Venskutoniene, Lina Katauskyte
Department of Crop Science and Animal Husbandry, Lithuanian University of Agriculture, Studentu 11, Akademija, Kaunas distr. LT-53067, Lithuania
*Corresponding author, E-mail: natalija@nora.lzua.lt


Linseed flax cultivars ‘Lirina’, ‘Barbara’ and ‘Szaphir’ were tested for their regeneration capacity through adventitious shoot organogenesis from hypocotyl-derived and stem segment-derived callus. Shoots number per explant was strongly influenced by genotype, culture medium and application of growth regulators. The highest shoot number per explant was obtained from hypocotyl-derived callus of cultivar ‘Szaphir’ on combined MSB5 media, supplemented by 2.0 mg l-1 N6-Δ2-isopentenyl adenine. Hypocotyl-derived callus gave the better results than stem segment-derived callus. Four week-old callus showed the maximum shoot regeneration frequency.

Key words: callus, genotype, growth regulators, linseed flax, shoot organogenesis.

Acta Univ Latv (2005) 691: 129–135
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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