Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2005) 691: 83–91
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Environmental and

Acta Univ Latv (2005) 691: 83–91

Orginal Article

The effect of stringent control on valine biosynthesis by Corynebacterium glutamicum

Ilze Deniņa, Longina Paegle, Maija Ruklisha*
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda Bulv. 4, Rīga LV-1586, Latvia
*Corresponding author, E-mail: coryne@lanet.lv


The present study focused on methods of stringent response induction and the investigations of its effect on valine synthesis by the isoleucine auxotroph Corynebacterium glutamicum 13032 ΔilvA pJC1ilvBNCD. The intracellular concentration of guanosine tetraphosphate increased and bacterial growth rate decreased, i.e. intracellular stringent response, was induced under conditions of (i) cell culture growth in medium lacking isoleucine and (ii) reduced isoleucine uptake by cells. The induction of the cellular stringent response was followed by an increase in the activity of acetoxydroxy acid synthase, e.g. ilvBN-encoded key enzyme of valine synthesis, and by a drastic increase in the cell specific rate of amino acid synthesis. It was demonstrated that induction of the stringent response can be used as method to increase valine overproduction by C. glutamicum cells.

Key words: Corynebacterium glutamicum, ppGpp, stringent control, valine biosynthesis.

Acta Univ Latv (2005) 691: 83–91
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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