Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2006) 710: 29–40
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Environmental and
Experimental
Biology

Acta Univ Latv (2006) 710: 29–40

Orginal Article

Effect of cultivation conditions on morphological and biochemical characteristics of lily explants in vitro

Gunta Jakobsone1*, Dace Megre2, Gederts Ievinsh1,2
1Tissue Culture Department, National Botanic Garden, Miera 1, Salaspils LV-2169, Latvia
2Department of Plant Physiology, Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Rīga V-1586, Latvia
*Corresponding author, E-mail: gunta.jakobsone@nbd.apollo.lv

Abstract

Regeneration of bulblets from excised bulbscales has become the preferred method for vegetative propagation of lilies. For optimal ex vitro growth and survival during in vitro propagation of lilies bulblets need to have both a period of high temperature as well as cold storage. The objective of the present experiments was to determine the optimal sugar concentration in the medium and duration of pre-cold storage period for lily explants of Asiatic lily clone ‘NS-94’ and Trompet lily cv. ‘Mezhare’ and to relate morphological characteristics with peroxidase and polyphenol oxidase activities. Morphological parameters of lily microplants were affected by cultivation conditions, e.a., low temperature storage, length of pretreatment period before cold treatment, as well as by light regime and sucrose concentration in the medium. Growth of bulblets was strongly affected by cold treatment, leading to a significant decrease of relative mass. Root formation was inhibited by cold treatment. This effect was more pronounced in cv. ‘Mezhare’ than clone ‘NS-94’. Formation of both leaves as well as bulblets was suppressed by cold storage of lily microplants. Cold treatment resulted in an increase of oxidative enzyme activity in bulblet tissues. Prolongation of duration of pre-cold period strongly increased peroxidase and polyphenol oxidase activity in bulblets of clone ‘NS94’, the effect was more pronounced in minimum light conditions. In contrast, for cv. ‘Mezhare’, higher enzyme activities were in bulblets of lily explants showing shortest duration of pre-cold period, especially in the light. A decrease in peroxidase activity appeared to be a good indicator for coldinduced slow growth of lily explants. In part this may be associated with increased antioxidative capacity in conditions of slow growth.

Key words: cold treatment, in vitro propagation, lily, morphological parameters, peroxidase, polyphenol oxidase.

 
Acta Univ Latv (2006) 710: 29–40
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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