Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2008) 745: 115–130
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Environmental and
Experimental
Biology

Acta Univ Latv (2008) 745: 115–130

Orginal Article

Expression of hepatitis C virus structural genes controlled by alphaviral recombinant replicons

Ekaterina Alekseeva*, Irina Sominskaya, Ruta Bruvere, Velta Ose, Anna Zajakina, Tatyana Kozlovska
Department of Protein Engineering, Latvian Biomedical Research and Study Centre,Ratsupites 1, Riga LV-1067, Latvia
*Corresponding author, E-mail: kate@biomed.lu.lv

Abstract

Since no highly efficient treatment for the hepatitis caused by hepatitis C virus (HCV) is available yet, the development of an anti-HCV vaccine candidate remains a goal of high priority. In our study, two different alphaviral expression systems were applied for expression of HCV structural protein genes in cell culture. For this purpose, the HCV genome fragment encoding the core-E1-E2-p7 polyprotein, and HCV core and E2 genes, separately, were cloned in Semliki Forest virus (pSFV1) and Sindbis virus (pCytTS) expression vectors. Efficient synthesis of structural HCV proteins in a BHK-21 cell line was demonstrated. HCV polyprotein precursor was authentically posttranslationally processed. Identification of HCV proteins was confirmed by immunoprecipitation, immunocytochemical analysis, and by Western blotting with corresponding anti-HCV antibodies.

Key words: alphavirus, expression, Hepatitis C virus, Semliki Forest virus, Sindbis virus.

 
Acta Univ Latv (2008) 745: 115–130
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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