Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2008) 745: 7–15
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Environmental and
Experimental
Biology

Acta Univ Latv (2008) 745: 7–15

Orginal Article

Subcellular localization of Hepatitis B virus core protein expressed from two different constructs in the Semliki Forest virus expression system

Sabine Braun1,2, Ruta Bruvere3, Anna Zajakina3*, Ekaterina Alekseeva3, Tatyana Kozlovska3
1Institut für Virologie, Charité, Humboldt-Universität, Universitätsklinikum Charité, Schumannstraße 20/21, 10117 Berlin, Germany
2Present address: Klinikum Augsburg, Institut für Laboratoriums-medizin, Mikrobiologie und Umwelthygiene, Stenglinstraße 2, 86156 Augsburg, Germany
3Department of Protein Engineering, Latvian Biomedical Research and Study Centre, Ratsupites 1, Riga LV-1067, Latvia
*Corresponding author, E-mail: anna@biomed.lu.lv

Abstract

The subcellular localization of Hepatitis B virus (HBV) core protein in eukaryotic cells was studied by an immunocytochemical method following expression of the HBV core (HBc) gene in the Semliki Forest virus expression system from two different constructs. HBc protein showed cytoplasmic and nuclear localization when it was expressed from a construct that encoded the HBV pregenome, whereas HBc protein that was expressed from a construct encoding the HBc gene alone was found only in the cytoplasm. The possible reasons for this phenomenon are discussed.

Key words: HBV core protein, HBV pregenome, Hepatitis B virus, Semliki Forest virus, subcellular localization.

 
Acta Univ Latv (2008) 745: 7–15
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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