Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2008) 745: 17–23
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Environmental and

Acta Univ Latv (2008) 745: 17–23

Orginal Article

Morphogenesis of wild orchid Dactylorhiza fuchsii in tissue culture

Gunta Jakobsone*
Tissue Culture Department, National Botanical Garden, Miera 1, Salaspils LV-2169 Latvia
*Corresponding author, E-mail: gunta.jakobsone@nbd.gov.lv


Dactylorhiza fuchsii which is included in the Red Data Book of the Baltic Region and listed in Annex B of the EC Habitats Directive was chosen as a model to study the initial development stages of asymbiotic development in tissue culture. Half-mature seeds were removed from sterilized seed capsules and sown onto filter-paper bridges in culture tubes with initial liquid medium and kept in the dark at 23°C. Germination started after two months of incubation. Protocorms formed were transplanted into fresh medium. Initiation of roots and tubers was observed after 3 to 6 months of germination. Plantlets with ~ 2 cm long shoots and well-developed roots were transferred into a light chamber. New plants of D. fuchsii with two leaves, tubers and roots appropriate for transplanting into soil were obtained in the next spring, e.g. about 8 to 9 months after the start of germination. The use of in vitro methods significantly reduced the relatively long period of time necessary for development of orchid plants in natural conditions.

Key words: asymbiotic development, Dactylorhiza fuchsii, morphogenesis, tissue culture.

Acta Univ Latv (2008) 745: 17–23
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
Published by
University of Latvia

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