Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2008) 745: 179–186
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Environmental and
Experimental
Biology

Acta Univ Latv (2008) 745: 179–186

Orginal Article

Growth of tissue culture and changes in oxidative enzyme activity of Sorbus and tayberry cultivars during cold storage

Dace Kļaviņa1*, Gederts Ievinsh2
1Tissue Culture Department, National Botanical Garden, Miera 1, Salaspils LV-2169 Latvia
2Department of Plant Physiology, Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Rīga LV-1586, Latvia
*Corresponding author, E-mail: dace.klavina@nbd.gov.lv

Abstract

The aim of the present work was to compare the effect of different concentrations of sucrose on success of long-term cold storage of two woody plant tissue cultures – Sorbus and tayberry cultivars. Increased sucrose concentration significantly diminished the number of necrotic shoots for Sorbus explants. High sucrose concentration in the medium together with other optimal cultivation conditions allowed successful storage of Sorbus and tayberry cultivars without subculturing for five years. After transplanting to fresh medium at 24°C explants completely renewed growth and development. During the period of initial growth inhibition at low temperature the activity of oxidative enzymes peroxidase and polyphenol oxidase increased, reaching a peak value when growth of the explants resumed. Increase of both peroxidase and polyphenol oxidase activity during the period of growth inhibition was positively correlated with the concentration of sucrose in the incubation medium.

Key words: cold storage, oxidative enzymes, Sorbus, sucrose, tayberry, tissue culture.

 
Acta Univ Latv (2008) 745: 179–186
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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