Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2009) 753: 107–115
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Acta Univ Latv (2009) 753: 107–115

Orginal Article

Alteration of serum adhesion molecules and cutaneous endothelium-dependent vasodilatation in insulin resistant obese patients

Inga Bormane1, Peteris Tretjakovs1,2,3*, Antra Jurka1,4, Indra Mikelsone1, Dace Reihmane1, Liga Balode1, Karlina Elksne1, Juris Imants Aivars2, Inga Stukena3, Kristine Cirule3, Guntis Bahs3, Valdis Pirags4
1Institute of Experimental and Clinical Medicine, University of Latvia, Ojara Vaciesa 4, Riga LV-1004, Latvia
2Department of Human and Animal Physiology, Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Riga LV-1586, Latvia
3Department of Human Physiology and Biochemistry, Riga Stradins University, Dzirciema 16, Riga LV-1007, Latvia
4Centre of Endocrinology, Pauls Stradins Clinical University Hospital, Pilsonu 13, Riga LV-1002, Latvia
*Corresponding author, E-mail: tretjako@latnet.lv


Insulin resistance (IR) is associated with decreased endothelium-dependent vasodilatation. Adhesion molecules are regarded as endothelial dysfunction biomarkers, which are markedly upregulated in obesity-linked diseases, including coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM). The purpose of the study was to evaluate the relationships between IR, vascular cell adhesion molecule-1 (sVCAM-1), intercellular cell adhesion molecule-1 (sICAM-1), sEselectin, and endothelium-dependent vasodilatation in metabolic syndrome (MetS) patients who were categorized as having T2DM, both T2DM and CAD, or neither. Obese MetS patients with dyslipidemia were classified into three groups: 34 patients with T2DM (D), 20 patients with T2DM and CAD (DC), and 26 patients with MetS alone (M). Eighteen healthy subjects were selected as controls (C). The study groups were matched for age and sex. IR was assessed by HOMA-IR method, and serum sVCAM-1, sICAM-1, and sE-selectin levels were measured by xMAP technology. Laser Doppler imaging with iontophoretic application of 1% acethylcholine (LDI-Ach) solution was used for the evaluation of cutaneous endothelium-dependent vasodilatation in the hand. Serum levels of sVCAM-1, sICAM-1, and sE-selectin were significantly higher in the group of DC patients compared with the levels in other groups (p < 0.01), except for sICAM-1 in the D group. Also, sVCAM-1, sICAM-1, and sE-selectin concentrations were significantly correlated with HOMA-IR indexes (p < 0.0001). Only D and DC patient groups demonstrated a significant and similar decline in LDI-Ach marker compared to the group of healthy subjects (p < 0.001). LDI-Ach values were significantly correlated with HOMA-IR indexes, sVCAM-1, sICAM-1, and sE-selectin levels (p < 0.01). Our findings show that obese MetS patients with T2DM have more higher serum levels of adhesion molecules (sICAM-1, sVCAM-1, and sE-selectin), simultaneously with both higher IR and lower endothelium-dependent vasodilatation than those with MetS alone, and the presence of CAD in these patients is associated with greater changes in the endothelial dysfunction markers. IR was observed to be a closely related to endothelial dysfunction.

Key words: adhesion molecule, endothelium-dependent vasodilatation, insulin resistance, metabolic syndrome.

Acta Univ Latv (2009) 753: 107–115
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh

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University of Latvia

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