Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2010) 8: 81–84
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Environmental and
Experimental
Biology

Env Exp Biol (2010) 8: 81–84

Orginal Article

High frequency of shoot regeneration on leaf explants of Bacopa monnieri

Aruna G. Joshi*, Ashutosh R. Pathak, Asha M. Sharma, Swati Singh
Department of Botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390 002, India
*Corresponding author, E-mail: arunajoshi@gmail.com

Abstract

Plants of Bacopa monnieri were regenerated from entire leaf explants on Murashige and Skoog’s basal medium supplemented with different concentrations (2 – 12 μM) of cytokinin 6-benzylaminopurine. The largest numbers of adventitious shoot buds were induced in 100% cultures from the explant when 6-benzylaminopurine was used at a concentration of 6 μM. The rate of adventitious shoot bud regeneration was affected by the type of wounding and placement of the explant on the medium. The square leaf lamina produced the maximum number of shoots within eight weeks. Rooting was achieved in microshoots on 1/2 strength basal liquid medium supplemented with sucrose (1%) and indole-3-butyric acid (2 μM). The obtained plantlets could be successfully acclimatized ex vitro.

Key words: leaf lamina explant, nodular callus, regeneration in vitro, shoot buds.

 
Env Exp Biol (2010) 8: 81–84
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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