Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2010) 8: 97–101
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Environmental and
Experimental
Biology

Env Exp Biol (2010) 8: 97–101

Brief Communication

Staphylococcus aureus sortase A cyclization and evaluation of enzymatic activity in vitro

Dmitry Zhulenkovs*, Ainars Leonchiks
Latvian Biomedical Research and Study Centre, Ratsupites 1, Riga LV-1067, Latvia
*Corresponding author, E-mail: dmitry@biomed.lu.lv

Abstract

Protein cyclization has been shown to increase the stability of molecule structure by reducing the number of possible conformation variations. Cyclization of an enzyme can significantly enlarge the spectrum of its application in specific conditions, including high temperature, presence of a chaotropic agent, and denaturing environment. In this study the cyclic form of Staphylococcus aureus sortase A enzyme (srtA) was obtained by introducing a peptide bond between amino acids at N- and C-termini. The catalytic activity of purified cyclic srtA was tested in vitro by protein-protein ligation reaction. In contrast to the linear srtA, the cyclic form of the enzyme was active in presence of chaotropic agent. This finding suggests the potential use of srtA enzyme in protein engineering techniques.

Key words: cyclization, sortase A, Staphylococcus aureus, transpeptidation, trans-splicing.

 
Env Exp Biol (2010) 8: 97–101
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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