Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2011) 9: 1–8
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Environmental and

Env Exp Biol (2011) 9: 1–8

Original Article

The influence of the land use on abundance and diversity of soil fungi: comparison of conventional and molecular methods of analysis

Lelde Grantina1*, Elina Seile1, Kristine Kenigsvalde1, Raimonds Kasparinskis2, Guntis Tabors3, Vizma Nikolajeva1, Pieter Jungerius4, Indrikis Muiznieks1
1Department of Microbiology and Biotechnology, Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Riga LV-1586, Latvia
2Department of Environmental Science, Faculty of Geography and Earth Sciences, University of Latvia, Alberta 10, Riga LV-1010, Latvia
3Department of Botany and Ecology, Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Riga LV-1586, Latvia
4Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
*Corresponding author, E-mail: lelde.grantina@lu.lv


This study examined fungal communities in 11 soil profiles. The objective was to apply conventional and molecular microbiology methods to obtain baseline information on the general characteristics of soil microbial communities in relation to land use – forest, former agricultural land, meadow and arable land. The number of filamentous fungi species and the total number of cultivable microorganisms showed a tendency to decrease with increasing depth. The diversity of fungi obtained with amplified rDNA gene restriction analysis was similar for all studied land use groups, diversity decreased with soil depth, especially in soils of former agricultural land and meadow. The proportion of fungal DNA as part of the total soil DNA was significantly higher in forest and abandoned land soil than in agricultural soil. The amount of Trichoderma spp. DNA was similar for all land groups, but its relative amount as percentage of total fungal DNA was higher in meadow and arable land soil. The land use type had a significant impact only on the diversity of cultivable soil fungi and fungal DNA amount. Soil depth and moisture content had a greater effects.

Key words: ARDRA, fungal diversity, Shannon-Weaver diversity index, soil, quantitative PCR, Trichoderma.

Env Exp Biol (2011) 9: 1–8
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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