Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2011) 9: 71–74
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Environmental and
Experimental
Biology

Env Exp Biol (2011) 9: 71–74

Brief Communications

In vitro cultivation and root initiation of the endangered plant Pulsatilla patens

Gunta Priede, Dace Kļaviņa*
National Botanical Garden of Latvia, Miera 1, Salaspils LV-2169, Latvia
*Corresponding author, E-mail: dace.klavina@nbd.gov.lv

Abstract

Pulsatilla patens (L.) Mill. is endangered due to habitat loss and degradation by various factors including heavy overgrowth of vegetation and a dense moss layer. In the present study, a suitable rooting medium for the difficult-to-root species P.&nbap;patens was developed on the basis of multistage rooting experiments. The best rooting was obtained in darkness for five days on a half-diluted Murashige-Skoog medium supplemented with 5 mg L–1 α-naphthaleneacetic acid and riboflavin followed by auxin photooxidation. It was concluded that, for in vitro formation of good quality roots on explants, it is important to provide exogenous auxins during root induction and to reduce auxin content during the expression stage. In vitro rooted plantlets of P. patens successfully acclimatized ex vitro in a peat substrate mixed with sand, and they developed well-branched roots and healthy leaf rosettes. After transplantation to semi-natural conditions, plants started to flower after a year.

Key words: auxins, ex situ conservation, in vitro propagation, plantlet establishment, Pulsatilla patens, rooting.

 
Env Exp Biol (2011) 9: 71–74
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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