Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2011) 9: 129–132
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Environmental and

Env Exp Biol (2011) 9: 129–132

Brief Communication

In vitro propagation of Citrus megaloxycarpa

Adhikarimayum Haripyaree1*, Kshetrimayum Guneshwor1, Huidrom Sunitibala2, Maibam Damayanti1
1Department of Life Sciences, Manipur University, Imphal, 795003, India
2Institute of Bioresources and Sustainable Development, Takyelpat, Manipur, India
*Corresponding author, E-mail: adhikarimayum_devi@yahoo.in


An efficient micropropagation protocol was established for Citrus megaloxycarpa Lush., a highly acidic citrus cultivar of Manipur, India, through shoot tip explant culture. The shoot tip explants produced multiple shoot buds when cultured on agarized Murashige and Skoog’s medium containing 0.25 to 2 mg L–1 N6 benzyl adenine (BA) alone, and in combination with 0.50 mg L–1 naphthalene acetic acid (NAA) or with 0.50 mg L–1 kinetin. The maximum number of shoots was induced on medium containing 0.25 mg L–1 BA together with 0.50 mg L–1 NAA or 1 mg L–1 BA with 0.50 mg L–1 kinetin. The separated shoots were rooted and elongated on medium containing 1 to 2 mg L–1 indole acetic acid, indole butyric acid and NAA. NAA was superior to other growth regulators for in vitro root induction. Axillary shoots were induced from in vitro raised plantlets by decapitating them. Axillary shoot tip explants were used for further multiple shoot bud induction. A maximum of about 120 plantlets were obtained from a single seedling. Rooted plantlets were successfully established in the soil. Efficient mass multiplication of this important fruit was achieved.

Key words: axillary shoot induction, Citrus megaloxycarpa, MS medium, shoot tip, multiple shoot induction.

Env Exp Biol (2011) 9: 129–132
 DOI: http://doi.org/10.22364/eeb

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