Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2011) 9: 75–81
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Environmental and
Experimental
Biology

Env Exp Biol (2011) 9: 75–81

Original Article

haumatin-like protein gene copy number variation in Scots pine (Pinus sylvestris)

Vilnis Šķipars, Baiba Krivmane, Dainis Ruņģis*
Latvian State Forest Research Institute “Silava” Genetic Resource Centre, Rigas 111, Salaspils LV-2169, Latvia
*Corresponding author, E-mail: dainis.rungis@silava.lv

Abstract

Copy number variations (CNVs) are structural variations in genomes, resulting from the duplication of a section of DNA that is usually from one kilobase to several megabases in size. The aim of this research was to investigate gene copy number variation in Scots pine (Pinus sylvestris). Twenty nine Scots pine trees from a research pine plantation were tested for CNVs of a thaumatin-like protein (TLP) gene, by utilising real-time PCR with TaqMan probes and a relative quantification approach. DNA quantitation results indicated that CNVs of the TLP gene were detected in the analysed samples. The results were confirmed by repeating the quantitation analysis using a different endogenous control. In addition, all members of an open-pollinated half sib family had increased copy numbers of the TLP gene, indicating the genetic basis of these results. The results presented in this paper are to our knowledge the first reported results of CNV polymorphism in a conifer species.

Key words: copy number variations, Pinus sylvestris L., real-time PCR, thaumatin-like protein.

 
Env Exp Biol (2011) 9: 75–81
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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