Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2012) 10: 1–7
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Environmental and

Env Exp Biol (2012) 10: 1–7

Original Article

Endophytic fungi in evergreen rhododendrons cultivated in vitro and in vivo

Liva Purmale1*, Inga Apine1, Vizma Nikolajeva2, Lelde Grantina2, Gerard Verkley3, Signe Tomsone1
1Plant Biology Laboratory, Botanical Garden, University of Latvia, Kandavas 2, Riga LV–1083, Latvia
2Department of Microbiology and Biotechnology, Faculty of Biology, University of Latvia, Kronvalda Bulv. 4, Riga LV–1010, Latvia
3Centraalbureau voor Schimmelcultures (CBS) / Fungal Biodiversity Centre, Uppsalalaan 8, NL 3584 CT Utrecht, The Netherlands
*Corresponding author, E-mail: liva.purmale@gmail.com


The aim of the study was to survey fungal endophytes inhabiting healthy leaves and stems of evergreen rhododendrons and during in vitro tissue culture for their propagation. Fungi were identified using morphological traits. The main taxa observed in rhododendron leaves in vivo were Phomopsis, Phoma, Alternaria spp., Colletotrichum, Cladosporium, Seimatosporium, Diplodina, Coleophoma, Cryptocline, Truncatella and Guignardia spp. Contamination in tissue culture were caused by Penicillium, Cladosporium, and Cephalosporium spp. The study supported the view that endophytes can infect medium of in vitro cultivated rhododendrons. Fungal diversity increased after acclimatization of rhododendron plantlets ex vitro. The presence of microorganisms in rhododendron cultivated in vitro depended both on age of tissue and host genotype.

Key words: endophytes, fungi, in vitro culture, rhododendrons.

Env Exp Biol (2012) 10: 1–7
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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