Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2013) 11: 59–67
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Environmental and
Experimental
Biology

Env Exp Biol (2013) 11: 59–67

Original Article

Micropropagation, in vitro flowering and cytological studies of Bacopa chamaedryoides, an ethno-medicinal plant

Sk Moquammel Haque, Biswajit Ghosh*
Plant Biotechnology Laboratory, Department of Botany, Ramakrishna Mission Vivekananda Centenary College, Rahara, Kolkata – 700118, India
*Corresponding author, E-mail: ghosh_b2000@yahoo.co.in

Abstract

This is the first report of both establishment of micropropagation protocol as well as cytological studies of Bacopa chamaedryoides (Kunth) Wettst., an important Indian ethno-medicinal herb. Shoot-tips and nodal segments explants were inoculated on Murashige and Skoog basal medium containing 0.7% agar, 3.0% sucrose and different concentrations and combinations of cytokinins and auxins. Optimum multiplication was achieved on medium containing 6-benzyl-aminopurine (2.0 mg L–1) and indole-3-acetic acid (0.2 mg L–1). Shoot-tips proved to be a better explant in having a high rate of shoot multiplication (18.7 ± 0.17) in comparison to nodal segments (15.1 ± 0.18) in the same medium. In vitro rooting of multiplied individual shoots was achieved on half strength Murashige and Skoog medium supplemented with 50% of ‘Aloe vera gel’, with a maximum of 18.3 ± 0.17 roots. Up to 66.7% of these multiplied shoots induced healthy flowers in vitro on Murashige and Skoog medium containing low concentration of 6-benzyl-aminopurine (0.2 mg L–1). In vitro produced flowers contained 96.54 % viable pollen, more or less same as the field grown mother plants. Micropropagated plants have shown normal diploid 2n = 22 chromosomes, same as that of the mother plant. These micropropagated plants were successfully established in soil after hardening them in submerged condition, with an 84% survival rate. In total, 88.9% of the survived plants flowered and fruited normally after 50 days of field transfer. More than 85% field-grown regenerated plants developed normal fruits and viable seeds. This work presents an efficient micropropagation method from node and shoot-tip culture for mass propagation and in vitro flowering of B. chamaedryoides.

Key words: A Aloe vera gel, auxins, Bacopa chamaedryoides, cytokinins, cytology, in vitro flowering, pollen viability.

 
Env Exp Biol (2013) 11: 59–67
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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