Env Exp Biol (2013) 11: 119

Original Article

Sterilization factors affect seed germination and proliferation of Achyranthes aspera cultured in vitro

Monokesh Kumer Sen^*1, M. Abu Hena Mostofa Jamal¹, Shamima Nasrin²

¹Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
²Plant Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, Bangladesh
^*Corresponding author, E-mail: monokeshsen@yahoo.com

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Abstract

Achyranthes aspera (Amaranthaceae) is a highly significant medicinal herb that is common in Bangladesh. The effect of five sterilizing substances (ethanol, mercuric chloride, Flugal, Nystatin, and sodium hypochlorite) on germination and development of explants of A aspera was evaluated. The percentage of contamination and germination, as well as seed color, growth pattern, and shootlet development were determined. Among the different sterilants, sodium hypochlorite solution application for 30 min was the most effective treatment. At higher concentration, all sterilizing agents showed maximum effect against microbiological contaminantion, although the survival percentage was low.

Key words: Achyranthes aspera, microbiological contamination, seeds, sterilizing substances, tissue culture.

Env Exp Biol (2013) 11: 119–123

DOI: http://doi.org/10.22364/eeb

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Faculty of Biology, University of Latvia
				Hard copy: ISSN 1691–8088 On-line: ISSN 2255–9582 Env Exp Biol (2013) 11: 119–123 DOI: https://doi.org/10.22364/eeb
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