Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2015) 13: 19–23
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Environmental and
Experimental
Biology

Environ Exp Biol (2015) 13: 19–23

Orginal Articles

Agrobacterium rhizogenes-mediated genetic transformation of Psammosilene tunicoides and identification of high saponin-yielding clones

Hongfeng Wang1, Shuai Gao1, Jaime A. Teixeira da Silva2, *, Guohua Ma3, **
1 Biotechnological Institute of Guangdong Academy of Forestry, Guangzhou 510520, P. R. China
2 P.O. Box 7, Miki-cho Post Office, Miki-cho, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan
3 Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, P. R. China
*Corresponding author, E-mail: jaimetex@yahoo.com
**Corresponding author, E-mail: magh@scib.ac.cn

Abstract

Psammosilene tunicoides (WC Wu et CY Wu), a medicinal plant, was transformed with Agrobacterium rhizogenes strain ATCC15834, resulting in development of hairy roots from the cut surface of leaves. In total, 36 hairy root clones demonstrating prolific rooting and ageotropism were isolated. Hairy roots from all 36 clones were cultured in B5 liquid medium. Each clone had a different saponin content. Among the 36 clones, highest saponin content of 0.7‰ was observed in A-23. PCR confirmed that rolB and rolC genes of the Ri plasmid of A. rhizogenes had been transferred and expressed successfully in the hairy roots of P. tunicoides A-23.

Key words: Agrobacterium rhizogenes, genetic transformation, hairy roots, Psammosilene tunicoides, saponins.

 
Environ Exp Biol (2015) 13: 19–23
 DOI: http://doi.org/10.22364/eeb
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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