Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2015) 13: 133–138
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Environmental and

Environ Exp Biol (2015) 13: 133–138

Original paper

Evaluation of morphological differences of breast cancer cells using various biological markers and the Feulgen method

Zane Simsone1,*, Talivaldis Freivalds1, Liga Harju1, Dita Gudra1, Iveta Kudaba2, 3, Inta Liepniece-Karele3, 4, Indulis Buikis1, Juris Berzins1
1Institute of Experimental and Clinical Medicine, University of Latvia, Ojara Vaciesa 4, Riga LV–1004, Latvia
2Riga East University Hospital, Oncology Centre of Latvia, Hipokrata 4, Riga LV–1006, Latvia
3University of Latvia Faculty of Medicine, Raina Bulv. 19, Riga LV–1586, Latvia
4Riga East University Hospital, Pathology Centre, Hipokrata 2, Riga LV–1006
*Corresponding author, E-mail: z.simsone@gmail.com


Cancer cell nuclei have undergone chromatin structure condensation and changes in shape, nucleolus volume and staining properties. Nevertheless, it has been suggested that cancer stem cells are responsible for resistance to anticancer treatment and tumour recurrence. The aim of the study was to evaluate morphological differences and distribution of DNA amount in luminal and triple negative breast cancer cell populations using various biological markers and the Feulgen method. Multiple cell surface antigens like CD44 (the cluster of differentiation 44), CD24 (the cluster of differentiation 24), aldehyde dehydrogenase (ALDH) and nucleus antigens like embryonic stem cell marker (Oct3/4) can be used to identify cancer stem cells. Strong expression of antibody CD44 and ALDH and positive expression of CD24 and Oct3/4 were seen in both breast cancer groups among morphologically different cells. Amount of DNA indicated variability of cells. The study showed similarity between development and behaviour of microcells and polyploid cells, and we suggest that polyploid cells are in a later phase of development after anticancer treatment.

Key words: biological markers, breast cancer, Feulgen method, microcells.

Environ Exp Biol (2015) 13: 133–138
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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