Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2015) 13: 177–182
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Environmental and

Environ Exp Biol (2015) 13: 177–182

Orginal Articles

Antioxidant enzyme activity and germination characteristics of different maize hybrid seeds during ageing

Cyrus Mansouri-Far1,2, Maryam Goodarzian-Ghahfarokhi1,3,*, Mohsen Saeidi1, Majid Abdoli4
1 Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
2 Faculty of Agricultural Science, Payam-e-Noor University, Karaj, Iran
3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University, Ahvaz, Iran
4 Young Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran
* Corresponding author, E-mail: goodarzian98@gmail.com


The aim of this study was evaluation of the effect of ageing on germination traits and activity of antioxidant enzymes in embryo of maize hybrid seeds and the role of these traits in protection of seed vigour. A two-factorial experiment was performed using six maize hybrids, and four accelerated ageing levels (non-aged, aged for 2, 4 and 6 days) at 40 °C temperature and 95% humidity. Mean germination time and electrolyte leakage significantly increased with ageing. Mean germination time and electrolyte leakage of hybrids SC260, SC370 and SC647 increased more than in the other hybrids. Also, antioxidant enzyme activity decreased significantly with ageing. The results indicated severe damage to the cell membrane and antioxidant enzyme acivity in the hybrids under ageing. In general, loss of viability and increase in electrolyte leakage reflected the seed deterioration during storage under accelerated ageing conditions.

Key words: antioxidant enzymes, electrolyte leakage, germination, maize embryo, seed ageing.

Environ Exp Biol (2015) 13: 177–182
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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