Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2015) 13: 169–175
About the Journal Retractions Open Access Author Guidlines Current Issue Archive
Environmental and

Environ Exp Biol (2015) 13: 169–175

Orginal Articles

Phytochemical analysis, antioxidant and antibacterial activities of various extracts from leaves and stems of Chrozaphora tinctoria

Javad Sharifi-Rad1, Seyedeh Mahsan Hoseini-Alfatemi2, Abdolhossein Miri1, Majid Sharifi-Rad3, Leila Soufi4, Marzieh Sharifi-Rad5, William N. Setzer6, Masoumeh Hoseini7, Mehdi Sharifi-Rad8,*, Mehrad Rokni9
1 Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
2 Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Department of Range and Watershed Management, Faculty of Natural Resources, University of Zabol, Iran
4 Guilan University of Medical Sciences, Rasht, Iran
5 Department of Chemistry, Faculty of Science, University of Zabol, Zabol 98615-538, Iran
6 Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA
7 Department of Food Science and Technology, Faculty of Agriculture, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
8 Zabol University of Medical Sciences, Zabol, Iran
9 Department of Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
* Corresponding author, E-mail: mehdi_sharifirad@yahoo.com


The purpose of this study was to evaluate the amounts of phenolics, flavonoids, and tannins, and to determine the antioxidant and antibacterial activities of aqueous, acetone, methanol, and ethanol extracts of stems and leaves of Chrozaphora tinctoria L. from Zabol, Iran. After collection and extraction of C. tinctoria leaves and stems, the total phenolic, flavonoid, and tannin concentrations were determined spectrophotometrically. The antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl radical-scavenging assay, iron-reducing-power assay, β-carotene-bleaching assay, and antioxidant-capacity assay. Antibacterial activity was determined using disc diffusion assay. The results of this study indicated that C. tinctoria leaf extracts had higher antioxidant activity than in stem extracts. Methanol and acetone extracts had greater antioxidant and antibacterial activity than aqueous extracts. C. tinctoria has antiradical and antimicrobial activity that may be useful in pharmacy and phytotherapy.

Key words: antioxidant activity, antimicrobial activity, Chrozaphora tinctoria, phenolics, tannins.

Environ Exp Biol (2015) 13: 169–175
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
Published by
University of Latvia

For Authors
Directory of Open Access Journals
Google Scholar
Thomson Reuters
CAB Abstracts
Last modifications: 2021.10.18-09:17

Print ISSN 1691-8088 – Online ISSN 2255-9582 Copyright © 2022 University of Latvia