Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2016) 14: 121–126
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Environmental and

Environ Exp Biol (2016) 14: 121–126

Orginal Articles

Molecular variability of Apple chlorotic leaf spot virus isolated in Belarus

P. Kuzmitskaya1*, V. Titok2, B. Anoshenko2, O. Urbanovich1
Institute of Genetics and Cytology of National Academy of Science of Belarus, 27 Akademicheskaya Str., Minsk 220072, Belarus
2 Central Botanical Garden of National Academy of Science of Belarus, 2c Surganov Str., Minsk 220012, Belarus
* Corresponding author, E-mail: p.kuzmitskaya@igc.by


Apple chlorotic leaf spot virus (ACLSV) is a harmful latent pathogen distributed worldwide in apple orchards. To evaluate the genetic diversity of this virus in Belarusian apple orchards, 850 bp genome fragments coding parts of replicase and movement protein open reading frames from five viral isolates from apple in Belarus were sequenced. High genetic variability was detected among isolates studied. The potential sites of recombination were identified in the partial sequences of the replicase and movement protein genes of ASLSV. The estimation of selection pressure revealed strong negative selection on the genome fragments obtained in this study and homologous to them ACLSV sequences from the GenBank database. Relationships between the geographic origin and the nucleotide identity of the fragments of ACLSV were not found.

Key words: Apple chlorotic leaf spot virus, molecular variability, selection pressure, potential recombination events.

Environ Exp Biol (2016) 14: 121–126
 DOI: http://doi.org/10.22364/eeb.14.17

Prof. Gederts Ievinsh
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