Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2017) 15: 1–5
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Environmental and

Environ Exp Biol (2017) 15: 1–5

Orginal Paper

Effect of cytokinins on shoot proliferation of silver birch (Betula pendula) in tissue culture

Elva Girgžde1, Ineta Samsone2*
1 Faculty of Biology, University of Latvia, Jelgavas 1, Rīga LV–1004, Latvia
2 Latvian State Forest Research Institute “Silava”, Rīgas 111, Salaspils LV–2169, Latvia
* Corresponding author, E-mail: ineta.samsone@silava.lv


Micropropagation can be used as an alternative method for vegetative propagation of mature birch trees in order to increase effectiveness of breeding. In this study the optimal concentration of synthetic cytokinin 6-benzylaminopurine and naturally occurring cytokinin zeatin were determined for shoot proliferation of birch genotype Bau-40-17. Woody Plant Medium supplemented with 6-benzylaminopurine or zeatin at three different concentrations was used. The results showed that Woody Plant Medium supplemented with 1.0 mg L−1 6-bezylaminopuryne gave the best results for shoot multiplication. In the case of natural cytokinin zeatin, 1.0 mg L–1 with addition of 0.1 mg L–1 kinetin gave the best results, but proliferation rate was significantly lower than with synthetic cytokinin. The presence of cytokinin in the medium affected adventitious root formation and resulted in change of pH, which may have caused a positive effect on shoot multiplication rate.

Key words: Betula pendula, cytokinins, shoot culture, shoot multiplication.

Environ Exp Biol (2017) 15: 1–5
 DOI: http://doi.org/10.22364/eeb.15.01

Prof. Gederts Ievinsh
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University of Latvia

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