Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2018) 16: 177–183
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Environmental and

Environ Exp Biol (2018) 16: 177–183

Orginal Paper

Characteristics of an endophytic microbial consortium and its impact on rhizosphere microbiota of barley

Dagnija Vecstaudža1, Māris Seņkovs2, Vizma Nikolajeva2*, Olga Mutere1
1 Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas 1, Riga LV-1004, Latvia
2 Faculty of Biology, University of Latvia, Jelgavas 1, Riga LV-1004, Latvia
* Corresponding author, E-mail: vizma.nikolajeva@lu.lv


Biological fertilizers are being developed on the basis of plant growth promoting microorganisms isolated from the plant rhizosphere, phyllosphere and endosphere. The aim of this study was to determine influence of a consortium of plant endophytes on rhizosphere microbiota in a greenhouse vegetation experiment with barley. Fifty-five bacterial and fungal strains were isolated from leaves, stems and roots of oilseed rape and barley. Twentty five of them did not inhibit seed germination. Most of them belonged to the genera Serratia, Enterobacter, Brevibacillus and Pseudomonas. Individual isolates demonstrated phosphate solubilization ability, nitrification potential and urease activity. Impact of the multi-strain microbial consortium on cultivable microbiota of rhizosphere was estimated in a 19-day greenhouse experiment with barley. Concentration of phosphate solubilizing and cellulolytic microorganisms in the rhizosphere was below the detection limit. The consortium increased the total number of bacterial colony-forming units but did not affect the number of actinobacterial and fungal colony-forming units. Interactions between species in the multi-strain consortium and between introduced and indigenous populations are discussed.

Key words: endophyte, microbial consortium, nitrification, phosphate solubilization, rhizosphere, urease.

Environ Exp Biol (2018) 16: 177–183
 DOI: http://doi.org/10.22364/eeb.16.17

Prof. Gederts Ievinsh
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University of Latvia

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