Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2021) 19: 67–72
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Environmental and

Environ Exp Biol (2021) 19: 67–72

Orginal Paper

Thymus numidicus: phenolic constituents, antibacterial, and antioxidant activities of butanolic extract

Farid Ait Kaki1, Rachid Benkiniouar1, Ahmed Touil1*, Ibrahim Demirtas2, Amina Merzoug3, Latifa Khattabi4
1 Laboratory of Natural Product from Plants and Organic Synthesis, Department of Chemistry, University of Mentouri-Constantine-1, Route ain El-Bey, Constantine, Algeria
2 Department of Chemistry, Faculty of Science, Çankiri Karatekin University, Çankiri, Turkey
3 Department of Science of Life, Abdelhafid. Boussouf University Center, Mila, Algeria
4 Laboratory of Biochemistry, National Center for Research in Biotechnology, Ali Mendjeli ville, Constantine, Algeria
* Corresponding author, E-mail: ahmedtouil@yahoo.fr


Thymus numidicus Poir. (Lamiaceae) is a native plant of northern Algeria and Tunisia. It is used in traditional medicine in Algeria to treat respiratory and digestive diseases, and as a spice in food preparations. This study aimed to characterize the chemical constituents and to evaluate antibacterial and antioxidant activities of 1-butanol (BuOH) extract of aerial parts of T. numidicus. A total of 15 phenolic compounds were identified using HPLC-TOF/MS analysis, among them eight phenolic acids, five flavonoids and a stilbenoid glycoside named polydatin, which were reported for the first time from the species. Antibacterial effects of BuOH extract were determined using the disc diffusion method against four bacteria strains, where it has displayed a weak activity against three of them. In addition, BuOH extract showed significant antioxidant activity, which was measured using 1,1-diphenyl-2-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays for free radical scavenging activity, and cupric ion reducing antioxidant capacity assay. Our results suggest the use of T. numidicus as a natural antioxidant in food and pharmaceutical industries.

Key words: antibacterial activity, antioxidant activity, phenolic compounds, Thymus numidicus.

Environ Exp Biol (2021) 19: 67–72
 DOI: http://doi.org/10.22364/eeb.19.06

Prof. Gederts Ievinsh
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