Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
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Environ Exp Biol (2021) 19: 81–88
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Environmental and
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Biology

Environ Exp Biol (2021) 19: 81–88

Orginal Paper

Therapeutic potential of L-arginine in a rat model of ovarian ischemia-reperfusion injury

Oly Banerjee1, Siddhartha Singh1, Ananya Bose1, Sudipta Kundu2, Maitrayee Banerjee3, Dibyendu Ray1, Bithin Kumar Maji1, Sandip Mukherjee1*
1 Department of Physiology, Serampore College, 9 William Carey Road, Serampore, Hooghly-712201, West Bengal, India
2 Department of Physiology, Kalka Dental College, Partapur By-Pass, Meerut, India
3 Department of Physiology, Krishnagar Government College, Nadia-741101, West Bengal, India
* Corresponding author, E-mail: sm_kdc@yahoo.co.in

Abstract

Ischaemia-reperfusion (I/R) injury is a serious problem subsequent to reperfusion treatment for ovarian torsion. The role of nitric oxide (NO) in ovarian I/R injury is debatable. The main focus of this study was to explore the protective role of L-arginine, a potent NO precursor, on ovarian I/R injury. Female Wistar rats were divided into three groups (n = 5). In the control group, only laparotomy was performed. In the I/R group, ischaemia and reperfusion were performed and no drug was given. In the I/R + arginine group, ischaemia was followed by reperfusion and 200 mg kg  L-arginine was injected 5 min before reperfusion. Concentration of malondialdehyde, NO and reduced glutathione, as well as activity of superoxide dismutase and catalase were analyzed. Hematoxylin and eosin-stained slides were microscopically examined for histological evaluation of the ovaries. Superoxide dismutase and catalase activity along with concentration of reduced glutathione and NO were significantly lower in the I/R group in comparison to the control group. Malondialdehyde concentration was significantly higher in the I/R group than in control group. These results were reversed with supplementation of L-arginine. Light microscopic examination revealed severe vascular congestion, edema, haemorrhage, and follicular degeneration in the ovary tissue. The extent of ovarian damage was much higher in the I/R group than in the I/R + L-arginine group. Treatment with L-arginine seems to have an ameliorating effect against oxidative stress in I/R injury in rat ovary. It considerably reduced the altered histological changes in the ovaries. Thus, it can be speculated that L-arginine might play a pivotal role as a potent therapeutic agent against ovarian torsion.

Key words: antioxidant enzymes, ischaemia–reperfusion, L-arginine, ovarian torsion, oxidative stress.

 
Environ Exp Biol (2021) 19: 81–88
 DOI: http://doi.org/10.22364/eeb.19.08
EEB

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Prof. Gederts Ievinsh
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