Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2016) 14: 183–198
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Environmental and
Experimental
Biology

Environ Exp Biol (2016) 14: 183–198

Review

Disinfection of explants for saffron (Crocus sativus) tissue culture

Jaime A. Teixeira da Silva1a, Dariusz Kulus2b, Xinhua Zhang3c, Songjun Zeng3d, Guohua Ma3e, Abel Piqueras4f
1 P. O. Box 7, Miki-cho post office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan
2UTP University of Science and Technology in Bydgoszcz, Department of Ornamental Plants and Vegetable Crops – Laboratory of Biotechnology, Bernardyńska St. 6, PL-85-029 Bydgoszcz, Poland
3Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, 510650, China
4Department of Plant Breeding. CEBAS-CSIC, PO Box 164, 30100 Espinardo, Murcia, Spain
* Corresponding author, E-mail: ajaimetex@yahoo.com; bdkulus@gmail.com; cxhzhang@scib.ac.cn; dzengsongjun@scib.ac.cn; emagh@scib.ac.cn; fpiqueras@cebas.csic.es

Abstract

The choice of explant and its preparation are most likely one of the most important biotic factors that determine the success of a tissue culture protocol when the explant is derived from ex vitro organs. This review assesses the disinfection protocols available for saffron (Crocus sativus L.; Iridaceae) explants in an attempt to ascertain the most suitable set of parameters that could ensure successful tissue culture in subsequent treatments. From a methodological perspective, two explant types are most commonly adopted in saffron biotechnology, namely the use of fresh field-grown shoots, or dormant corms. The latter is more recommended to minimize contamination and to allow the use of more aggressive disinfection treatments.

Key words: corms, Crocus sativus, hydrogen peroxide, mercury II chloride, saffron, sodium hypochlorite, sterile distilled water, tissue culture.

 
Environ Exp Biol (2016) 14: 183–198
 DOI: http://doi.org/10.22364/eeb.14.25
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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