Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2019) 17: 169–177
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Environmental and
Experimental
Biology

Environ Exp Biol (2019) 17: 169–177

Orginal Paper

In vitro regeneration and propagation from fasciated stems of Vitex rotundifolia

Hanzhi Liang1,2, Yuping Xiong1,4, Beiyi Guo1,4, Haifeng Yan5, Shuguang Jian1, Hai Ren1, Xinhua Zhang1, Yuan Li1, Songjun Zeng1, Kunlin Wu1, Feng Zheng1, Jaime A. Teixeira da Silva3*, Youhua Xiong2*, Guohua Ma1*
1 Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, 510650, China
2 College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 264000, China
3 P.O. Box 7, Miki-cho Post Office, Miki-cho, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan
4 University of Chinese Academy of Sciences, Beijing 100039, China
5 Cash Crop Institute of Guangxi Academy of Agricultural Sciences, Nanning, 530007, China
* Corresponding author, E-mail: jaimetex@yahoo.com; youhuachina@126.com; magh@scib.ac.cn

Abstract

An efficient plant proliferation and regeneration system via fasciated stems was established for the first time in Vitex rotundifolia L. Aseptic nodal segments were used as explants and cultured on Murashige and Skoog medium supplemented with different plant growth regulators alone and in different combinations to investigate the induction, proliferation, axillary shoot initiation and elongation from fasciated stems and subsequent regeneration. The obtained results show that the growth of fasciated stems can be regulated by cytokinins. A higher concentration of cytokinins induced both the formation of axillary shoots from normal stems and the development of fasciated stems. Anatomical analyses revealed that fasciated stems (5 to 25 mm wide) were much wider than normal in planta shoot stems (2 to 3 mm wide). The pith cells of fasciated stems developed laterally to 0.05 – 0.08 mm in width, while normal stems were usually 0.02 to 0.04 mm in width. Flow cytometry indicated no obvious changes in chromosomes among the four types of shoots. This is the first report on the development of axillary shoots from fasciated stems in V. rotundifolia tissue culture. Our protocol serves as a new form of clonal plant regeneration.

Key words: axillary shoots, fasciated stem, anatomical analysis, flow cytometer, rooting, Vitex rotindifolia.

 
Environ Exp Biol (2019) 17: 169–177
 DOI: http://doi.org/10.22364/eeb.17.17
EEB

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Prof. Gederts Ievinsh
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University of Latvia

 
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