Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2019) 17: 185–191
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Environmental and
Experimental
Biology

Environ Exp Biol (2019) 17: 185–191

Orginal Paper

Inhibitory effect of essential oils from Pulicaria mauritanica and Micromeria debilis on growth of Alternaria spp., the causal agent of tomato early blight

Meryem Yakoubi1*, Nisserine Hamini-Kadar1, Mohammed Gherib2, Abdelilah Amrouche2, Wassim Yezli1 , Soumaya Lalia Benichou1, Mabrouk Kihal1
1 Laboratory of Applied Microbiology, Departement of Biology, Faculty of Nature and Life Sciences, Oran 1 University, Ahmed Ben Bella 1524, El M’Naouer 31000, Oran, Algeria
2 Laboratory of Microbiology, Department of Nature and Life Sciences, University Center Salhi Ahmed, Nâama B.P 66, Algeria
* Corresponding author, E-mail: meriembio@hotmail.com

Abstract

The aim of the present study was the evaluation of antifungal activity of essential oil (EO) extracted from two medicinal and aromatic plants (Pulicaria mauritanica and Micromeria debilis) as biological treatment for Alternaria spp., a pathogenic mould that causes early blight on tomato plants. EO was extracted by hydrodistillation and antifungal activity was analyzed in vitro using direct contact on agar against ten isolates of Alternaria spp. Extraction yield was 0.30 and 0.045% for P. mauritanica and M. debilis, respectively. Both EOs had inhibitory effect against mycelial growth of all studied isolates. However, the antifungal effect of EO isolated from P. mauritanica was stronger than for that from M. debilis, with 100% inhibition against Alternaria tenuissema (isolates A18 and A57), Alternaria alternata (isolate AT4), Alternaria solani (A37) and Alternaria tomatophila (AT01), when used at 1.1 µL mL–1 concentration. EO from M. debilis had 100% inhibition against isolates A42 and A57 of A. solani and A. tenuissema when used at 1.1 µL mL–1 concentration. The antifungal activity of the EOs might due to the synergic effects of their chemical compounds, mainly monoterpenes and sesquiterpenes.

Key words: Alternaria spp., antifungal activity, essential oils, medicinal and aromatic plants, Micromeria debilis, Pulicaria mauritanica.

 
Environ Exp Biol (2019) 17: 185–191
 DOI: http://doi.org/10.22364/eeb.17.19
EEB

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Prof. Gederts Ievinsh
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