Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2018) 16: 307–314
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Environmental and
Experimental
Biology

Environ Exp Biol (2018) 16: 307–314

Orginal Paper

Genetic differentiation of Phoma sp. isolates using retrotransposon-based iPBS assays

Vilnis Šķipars1*, Maryna Siaredzich2, Viktorija Belevich1, Natālija Bruņeviča1, Lauma Brūna1, Dainis E. Ruņģis1
1 Latvian State Forest Research Institute „Silava”, Rīgas 111, Salaspils LV–2169, Latvia
2 Forest Protection and Wood Science Department, Belarusian State Technological University, Sverdlova 13A, Minsk 220006, Belarus
* Corresponding author, E-mail: vilnis.skipars@silava.lv

Abstract

Phoma blight is a disease affecting Norway spruce, Scots pine and other conifer seedlings in many forest tree nurseries throughout the world. Members of the Phoma genus, the causatives of this disease, are difficult to distinguish morphologically and genetically. In this study the use of a retrotransposon-based polymerase chain reaction approach using iPBS amplification for intra-species genetic discrimination between Phoma samples is described. Eight retrotransposon-based iPBS primers were used to genotype DNA from pure cultures of several Phoma species. The utilised markers were able to discriminate between Phoma species, but not all of them were able to differentiate all Phoma sp. isolates investigated. Belarusian samples were found to be distinct from the Latvian Phoma isolates. The Belorussian isolates were very similar to each other. A combination of three iPBS markers (2001, 2076 and 2242) enabled partial differentiation of the investigated Belarusian Phoma isolates.

Key words: genetic discrimination, inter primer binding site (iPBS) markers, Phoma sp.

 
Environ Exp Biol (2018) 16: 307–314
 DOI: http://doi.org/10.22364/eeb.16.22
EEB

Editor-in-Chief
Prof. Gederts Ievinsh
Published by
University of Latvia

 
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