Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2017) 15: 257–274
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Environmental and
Experimental
Biology

Environ Exp Biol (2017) 15: 257–274

Orginal Paper

Transcriptomic response to methyl jasmonate treatment of Scots pine (Pinus sylvestris) seedlings

Krista Kānberga-Siliņa1, Elza Rauda1, Vilnis Šķipars1, Adam Vivian-Smith2, Igor Yakovlev2, Baiba Krivmane1, Ilze Šņepste1, Dainis Ruņģis1*
1 Genetic Resource Centre, Latvian State Forest Research Institute “Silava”, Rigas 111, Salaspils LV–2169, Latvia
2 Norwegian Institute of Bioeconomy Research, Section Forest Genetics and Biodiversity, Høgskoleveien 8, 1433 Ås, Norway
* Corresponding author, E-mail: dainis.rungis@silava.lv

Abstract

Scots pine (Pinus sylvestris L.) has high ecological and economic importance in Latvia. Induced resistance can protect trees from pathogen attack, and has potential for sustainable pest management. The effect of methyl jasmonate (MeJA) on the gene expression profile of Scots pine was determined in order to assess its potential to induce resistance. Two year old Scots pine ramets were treated with 10 mM MeJA and needle samples were collected two weeks after treatment. RNA was isolated from samples and transcription profiling was performed using the Ion Torrent PGM platform, and RT-PCR was used to confirm expression profiles for selected genes. Gene expression profiles results were summarized by a gene network analysis using gene functionality annotations. Results revealed that MeJA treatment diverts metabolic functions from growth and development to defensive pathways. Analysis of the transcriptome indicated that the MeJA treated Scots pine clone had an altered gene expression profile two weeks after the treatment, suggesting that the induction of defence mechanisms was still active at this time. The organism is in an induced state at least two weeks after the treatment, which confirms the potential of the MeJA use to induce resistance responses in Scots pine trees.

Key words: gene profiles. induced resistance, methyl jasmonate, next generation sequencing, Scots pine.

 
Environ Exp Biol (2017) 15: 257–274
 DOI: http://doi.org/10.22364/eeb.10.22364/eeb.15.27
EEB

Editor-in-Chief
Prof. Gederts Ievinsh



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University of Latvia

 
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