Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2021) 19: 141–149
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Environmental and
Experimental
Biology

Environ Exp Biol (2021) 19: 141–149

Orginal Paper

Screening and optimization of indole-3-acetic acid production by bacterial strain isolated from rice rhizosphere and its effects on plant growth

Biyas Mukherjee1,2, Sanchali Roy1, Ankita Dhara1, Sikha Dutta1*
1 Molecular Plant Pathology and Fungal Biotechnology Laboratory, Department of Botany, The University of Burdwan, Purba Bardhaman 713104, West Bengal, India
2 Department of Botany, East Calcutta Girls’ College, P 237, Lake Town Road, Block B, Sreebhumi, Lake Town, Kolkata 700089, West Bengal, India
* Corresponding author, E-mail: sikha.bu.academia@gmail.com

Abstract

The present study deals with the isolation of plant growth-promoting bacterial strains from rhizospheric soil collected from a rice field of Purba Bardhaman District, West Bengal, India. Among the isolated five strains, A5 was the best-performing strain as it had, plant growth promoting traits like, production of indole‑3‑acetic acid (IAA), siderophore, hydrogen cyanide and exopolysaccharides, ammonia, phosphate solubilization, nitrogen fixation etc. Strain A5, identified as Bacillus xiamensis by phenotypic characters and 16S rDNA sequence-based homology, was able to produce a copious amount of IAA, particularly in the case of 42‑h culture with 1.5% L‑tryptophan as a precursor. Media optimization with different carbon and nitrogen sources was conducted for maximum production of IAA. Strain A5 used fructose and casamino acid most efficiently as carbon and nitrogen sources, respectively. Growth parameters were increased in A5‑treated seedlings of mung bean compared to control seedlings. Considering the observed traits, strain A5 can definitely be considered as a novel plant growth-promoting bacterial strain that may serve very well as a biofertilizer in agricultural fields.

Key words: Bacillus xiamensis, indole‑3‑acetic acid, plant growth-promoting rhizobacteria, plant growth promoting traits.

 
Environ Exp Biol (2021) 19: 141–149
 DOI: http://doi.org/10.22364/eeb.19.14
EEB

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Prof. Gederts Ievinsh
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University of Latvia

 
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