Faculty of Biology, University of Latvia
EEB
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2021) 19: 173–183
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Environmental and
Experimental
Biology

Environ Exp Biol (2021) 19: 173–183

Orginal Paper

Amelioration of ethanol intoxicated ovarian dysfunction by Alocasia indica tuber: possible involvement of nuclear factor kappa B

Swagata Pal1, Ankita Bhattacharjee2, Oly Banerjee2, Shilpi K. Prasad2, Siddhartha Singh2, Sandip Mukherjee2*
1 Department of Physiology, Raja Peary Mohan College, Uttarpara, Hooghly 712258, West Bengal, India
2 Department of Physiology, Serampore College, Hooghly 712201, West Bengal, India
* Corresponding author, E-mail: sm_kdc@yahoo.co.in

Abstract

The protective efficacy of ethanolic extract of Alocasia indica tuber was studied on ethanol-intoxicated ovaries of rats, searching for the possible involvement of expression levels of nuclear factor kappa B (NF‑κB) and caspase‑3. The extract was prepared by 80% ethanol using Soxhlet. Female albino rats were intraperitoneally injected by ethanol (3 g kg‑1 body weight per day) with and without extract (200 and 400 mg kg‑1 body weight per day) for a period of 14 days. Oxidative stress parameters and the gonadal hormone profile were determined, and apoptosis assay and immuno-staining of NF‑κB and caspase‑3 were conducted. Oxidative stress parameters and antioxidant enzyme levels were regained by 41 to 90% and 36 to 96%, respectively, by extract at a higher dose in ethanol intoxicated rat. Decreased levels (18 to 28%) of follicle stimulating hormone, luteinizing hormone, estrogen and progesterone level due to ethanol intoxication were restored (19 to 98%) by extract at same dose, whereas elevated level of prolactin and corticosterone level were restored by 51 to 81%. The extract reduced number of apoptotic dead cells in the ovary with altered expression of NF‑κB and caspase‑3, indicated by lower number of positively stained cells in extract-treated rats. It appears that ethanolic extract of A. indica can suppress ethanol-induced ovarian apoptotic cell death and other reproductive changes.

Key words: Alocasia indica, apoptosis, caspase‑3, ethanol toxicity, ovary, NF‑κB.

 
Environ Exp Biol (2021) 19: 173–183
 DOI: http://doi.org/10.22364/eeb.19.17
EEB

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Prof. Gederts Ievinsh
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University of Latvia

 
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