Acta Univ Latv (2004) 676: 207–212

In vitro propagation of Vaccinium species

Abstract

The aim of the work was direct regeneration of shoots from meristems, as well as adventitious shoot production from leaves for regeneration and effective production of highbush blueberry Vaccinium corymbosum L. and lingonberry Vaccinium vitis-idaea L. The results showed that the shoot proliferation intensity of individual cultivars differed. Therefore, optimisation of cytokinin type and concentration for each cultivar was necessary. For meristem regeneration, improved multiplication was achieved on medium with zeatin in comparison with N6-Δ2-isopentenyl adenine. Thidiazuron was effective in adventitious shoot regeneration from leaf tissue of highbush blueberry, and both thidiazuron and zeatin for lingonberry leaf tissue. Microshoot rooting was achieved on Anderson culture medium supplemented with indole-3-butyric acid or directly in peat after dipping of shoots into indole-3-butyric acid solution under ex vitro conditions.