Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2005) 691: 137–143
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Environmental and

Acta Univ Latv (2005) 691: 137–143

Orginal Article

Induction of somatic embryos on in vitro cultured zygotic embryos of spring Brassica napus

Natalija Burbulis*, Ramune Kupriene
Department of Crop Science and Animal Husbandry, Lithuanian University of Agriculture, Studentu 11, Akademija, Kaunas distr. LT-53067, Lithuania
*Corresponding author, E-mail: natalija@nora.lzua.lt


Mature and immature zygotic embryos of three Brassica napus doubled haploid lines were examined for their ability to produce somatic embryos. Seedlings from mature seeds did not produce somatic embryos in all of the tested media. Induction of somatic embryogenesis directly from immature seeds of doubled haploid lines was obtained on plant growth regulator-free medium. There was no callus phase, thus, the time of induction was very short. Age of zygotic embryos was the most significant factor influencing somatic embryos induction and development into plantlets. Immature zygotic embryos at the age of 20 to 21 days after pollination were most suitable for direct somatic embryogenesis in the tested rapeseed doubled haploid lines.

Key words: Brassica napus, zygotic embryos, somatic embryogenesis.

Acta Univ Latv (2005) 691: 137–143
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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