Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Acta Univ Latv (2006) 710: 131–138
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Environmental and

Acta Univ Latv (2006) 710: 131–138

Orginal Article

Comparison of anther culture response among Linum usitatissimum L. cultivars and their hybrids

Natalija Burbulis*, Ausra Blinstrubiene
Laboratory of Genetic and Biotechnology, Faculty of Agronomy, Lithuanian University of Agriculture, Studentu 11, Akademija, Kaunas distr. LT-53361, Lithuania
*Corresponding author, E-mail: natalija.burbulis@lzuu.lt


Linseed (Linum usitatissimum L.) is an important crop in Lithuania for production of both oil and fibre, but a Lithuanian cultivar of linseed has not previously been developed yet. Identification of responsive genotypes and development of efficient culture protocols are a prerequisite to initiating an effective doubled haploid production system in applied breeding programme. Anther culture response in L. usitatissimum was studied in the hybrid populations from responsive and poor/nonresponsive cultivars and their parental forms. Two different growth regulators combinations and three concentrations of sucrose were studied. Variation for anther culture response was significant both within and between the hybrids and their parents. F1 hybrids, produced from crosses between responsive genotype ‘Lirina’ and poor/non-responsive genotype ‘Barbara’, showed dramatic increases in callus induction in comparison with the parental form on medium supplemented by 1mg l-1 6-benzylaminopurine and 2 mg l-1 2,4-dichlorophenoxyacetic acid containing 6 % sucrose.

Key words: anther culture response, cultivars, hybrids, linseed.

Acta Univ Latv (2006) 710: 131–138
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
Published by
University of Latvia

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